Food sensing: selection and characterization of DNA aptamers to Alicyclobacillus spores for trapping and detection from orange juice.
Identifieur interne : 000644 ( Main/Exploration ); précédent : 000643; suivant : 000645Food sensing: selection and characterization of DNA aptamers to Alicyclobacillus spores for trapping and detection from orange juice.
Auteurs : Tim Hünniger [Allemagne] ; Christin Fischer ; Hauke Wessels ; Antonia Hoffmann ; Angelika Paschke-Kratzin ; Ilka Haase ; Markus FischerSource :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2015.
English descriptors
- KwdEn :
- Alicyclobacillus (genetics), Alicyclobacillus (isolation & purification), Aptamers, Nucleotide (genetics), Beverages (analysis), Beverages (microbiology), Biosensing Techniques (instrumentation), Biosensing Techniques (methods), Citrus sinensis (microbiology), Food Contamination (analysis), SELEX Aptamer Technique (instrumentation), SELEX Aptamer Technique (methods), Spores, Bacterial (genetics), Spores, Bacterial (isolation & purification).
- MESH :
- chemical , genetics : Aptamers, Nucleotide.
- analysis : Beverages, Food Contamination.
- genetics : Alicyclobacillus, Spores, Bacterial.
- instrumentation : Biosensing Techniques, SELEX Aptamer Technique.
- isolation & purification : Alicyclobacillus, Spores, Bacterial.
- methods : Biosensing Techniques, SELEX Aptamer Technique.
- microbiology : Beverages, Citrus sinensis.
Abstract
The quality of the beverage industry's products has to be constantly monitored to fulfill consumers' high expectations. The thermo-acidophilic Gram-positive Alicyclobacillus spp. are not pathogenic, but their heat-resistant endospores can survive juice-processing conditions and have become a major economic concern for the fruit juice industry. Current detection methods rely on cultivation, isolation, and organism identification, which can take up to a week, resulting in economic loss. This work presents the selection and identification of DNA aptamers targeting Alicyclobacillus spores by spore-SELEX (systematic evolution of ligands by exponential enrichment) in orange-juice-simulating buffer. The selection process was verified by various techniques, including flow cytometric binding assays, radioactive binding assays, and agarose gel electrophoresis. The subsequent aptamer characterization included the determination of dissociations constants and selectivity by different techniques, such as surface plasmon resonance spectroscopy and fluorescence microscopy. In summary, 10 different aptamers with an affinity to Alicyclobacillus spp. have been developed, analyzed, and characterized in terms of affinity and specificity.
DOI: 10.1021/jf505996m
PubMed: 25639310
Affiliations:
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first="Christin" last="Fischer">Christin Fischer</name></author><author><name sortKey="Wessels, Hauke" sort="Wessels, Hauke" uniqKey="Wessels H" first="Hauke" last="Wessels">Hauke Wessels</name></author><author><name sortKey="Hoffmann, Antonia" sort="Hoffmann, Antonia" uniqKey="Hoffmann A" first="Antonia" last="Hoffmann">Antonia Hoffmann</name></author><author><name sortKey="Paschke Kratzin, Angelika" sort="Paschke Kratzin, Angelika" uniqKey="Paschke Kratzin A" first="Angelika" last="Paschke-Kratzin">Angelika Paschke-Kratzin</name></author><author><name sortKey="Haase, Ilka" sort="Haase, Ilka" uniqKey="Haase I" first="Ilka" last="Haase">Ilka Haase</name></author><author><name sortKey="Fischer, Markus" sort="Fischer, Markus" uniqKey="Fischer M" first="Markus" last="Fischer">Markus Fischer</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25639310</idno><idno type="pmid">25639310</idno><idno 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Hamburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg , Grindelallee 117, 20146 Hamburg</wicri:regionArea><wicri:noRegion>20146 Hamburg</wicri:noRegion><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName></affiliation></author><author><name sortKey="Fischer, Christin" sort="Fischer, Christin" uniqKey="Fischer C" first="Christin" last="Fischer">Christin Fischer</name></author><author><name sortKey="Wessels, Hauke" sort="Wessels, Hauke" uniqKey="Wessels H" first="Hauke" last="Wessels">Hauke Wessels</name></author><author><name sortKey="Hoffmann, Antonia" sort="Hoffmann, Antonia" uniqKey="Hoffmann A" first="Antonia" last="Hoffmann">Antonia Hoffmann</name></author><author><name sortKey="Paschke Kratzin, Angelika" sort="Paschke Kratzin, Angelika" uniqKey="Paschke Kratzin A" first="Angelika" last="Paschke-Kratzin">Angelika Paschke-Kratzin</name></author><author><name sortKey="Haase, Ilka" sort="Haase, Ilka" uniqKey="Haase I" first="Ilka" last="Haase">Ilka Haase</name></author><author><name sortKey="Fischer, Markus" sort="Fischer, Markus" uniqKey="Fischer M" first="Markus" last="Fischer">Markus Fischer</name></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alicyclobacillus (genetics)</term><term>Alicyclobacillus (isolation & purification)</term><term>Aptamers, Nucleotide (genetics)</term><term>Beverages (analysis)</term><term>Beverages (microbiology)</term><term>Biosensing Techniques (instrumentation)</term><term>Biosensing Techniques (methods)</term><term>Citrus sinensis (microbiology)</term><term>Food Contamination (analysis)</term><term>SELEX Aptamer Technique (instrumentation)</term><term>SELEX Aptamer Technique (methods)</term><term>Spores, Bacterial (genetics)</term><term>Spores, Bacterial (isolation & purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Aptamers, Nucleotide</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Beverages</term><term>Food Contamination</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Alicyclobacillus</term><term>Spores, Bacterial</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term><term>SELEX Aptamer Technique</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Alicyclobacillus</term><term>Spores, Bacterial</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term><term>SELEX Aptamer Technique</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Beverages</term><term>Citrus sinensis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The quality of the beverage industry's products has to be constantly monitored to fulfill consumers' high expectations. The thermo-acidophilic Gram-positive Alicyclobacillus spp. are not pathogenic, but their heat-resistant endospores can survive juice-processing conditions and have become a major economic concern for the fruit juice industry. Current detection methods rely on cultivation, isolation, and organism identification, which can take up to a week, resulting in economic loss. This work presents the selection and identification of DNA aptamers targeting Alicyclobacillus spores by spore-SELEX (systematic evolution of ligands by exponential enrichment) in orange-juice-simulating buffer. The selection process was verified by various techniques, including flow cytometric binding assays, radioactive binding assays, and agarose gel electrophoresis. The subsequent aptamer characterization included the determination of dissociations constants and selectivity by different techniques, such as surface plasmon resonance spectroscopy and fluorescence microscopy. In summary, 10 different aptamers with an affinity to Alicyclobacillus spp. have been developed, analyzed, and characterized in terms of affinity and specificity.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Hambourg</li></region><settlement><li>Hambourg</li></settlement></list><tree><noCountry><name sortKey="Fischer, Christin" sort="Fischer, Christin" uniqKey="Fischer C" first="Christin" last="Fischer">Christin Fischer</name><name sortKey="Fischer, Markus" sort="Fischer, Markus" uniqKey="Fischer M" first="Markus" last="Fischer">Markus Fischer</name><name sortKey="Haase, Ilka" sort="Haase, Ilka" uniqKey="Haase I" first="Ilka" last="Haase">Ilka Haase</name><name sortKey="Hoffmann, Antonia" sort="Hoffmann, Antonia" uniqKey="Hoffmann A" first="Antonia" last="Hoffmann">Antonia Hoffmann</name><name sortKey="Paschke Kratzin, Angelika" sort="Paschke Kratzin, Angelika" uniqKey="Paschke Kratzin A" first="Angelika" last="Paschke-Kratzin">Angelika Paschke-Kratzin</name><name sortKey="Wessels, Hauke" sort="Wessels, Hauke" uniqKey="Wessels H" first="Hauke" last="Wessels">Hauke Wessels</name></noCountry><country name="Allemagne"><region name="Hambourg"><name sortKey="Hunniger, Tim" sort="Hunniger, Tim" uniqKey="Hunniger T" first="Tim" last="Hünniger">Tim Hünniger</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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